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KMID : 0379119980260020173
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Korean Journal of Mycology
1998 Volume.26 No. 2 p.173 ~ p.181
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Identification of Varieties by Biochemical Methods in Pleurotus spp.
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김경수/Kim, Kyung Soo
김영호/김동현/김영배/유창현/공원식/Kim, Young Ho/Kim, Dong Hyun/Kim, Young Bae/You, Chang Hyun/Kong, Won Sik
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Abstract
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To identify genetic difference of 13 strains in three Pleurotus species, analyses of rDNA, AP-PCR and RFLP were carried out. IGRI and ITSI∼II regions of rDNA amplifid by PCR were about 0.9 and 0.7 kb, respectively. These PCR products were digested with six restriction enzymes to analyse polymorphism. Especially, treatment of HaeIII enzyme on ITSI∼II regions showed specific bands in three Pleurotus sajor-caju strains. Genetic differences among three species were classified by similarity analyses based on rDNA polymorphism. Various band patterns of 2,500∼150 bp were showed by AP-PCR. Identification of species and varieties in 13 Pleurotus strains was possible according to primers used in AP-PCR. In order to develop genetic markers, RFLPs using IGRI and ITSI∼II probes derived from ASI 2180 and 2070 were carried out on eight Pleurotus varieties. RFLP patterns using IGRI probe were more various than that of ITSI∼II probe.
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